Applications

Our products offer the highest precision and sensitivity possible. Learn which products fit your needs best.

High-Throughput Gene Expression

Precise™ assays are an enabling technology for gene expression analysis. Precise assays offer qPCR users with a higher throughput, higher content, and more precise quantitative tool for profiling hundreds of genes in hundreds to thousands of samples. The inclusion of Molecular Indexes corrects for PCR bias and allows users to determine the absolute transcript counts for genes of interest. Precise assays also offer improvements and upgrades for gene expression applications via standard RNA-seq. The custom generated assays, streamlined library generation methods and data analysis tools offer a simple, turnkey solution without requiring advanced RNA-seq experience. This targeted approach is ideal for validating panels of genes of interest with the option of increasing sample throughput by an order of magnitude without adding additional costs. The high content of Precise assays and their ability to quantify even lowly expressed genes make it a valuable tool for quantitatively profiling complete pathways.

  • Ideal applications: Gene Expression validation, RNA-seq validation, pathway validation, rare transcript detection
  • Sample throughput: High
  • Targets per sample: 10 to 130
  • NGS compatibility: Compatible with all Illumina sequencers
  • Equipment required: Standard 96- or 384-well thermal cycler, no other equipment required; Pixel system is optional (Absolute Efficiency Standards)

High-Throughput RNA-seq

Single cells

  • Sample throughput: High
  • Targets per sample: 10 to 130
  • NGS compatibility: Compatible with all Illumina Sequencers
  • Equipment required: Standard 96- or 384-well thermal cycler, no other equipment required; Pixel system is optional (Absolute Efficiency Standards)

FFPE/Exosome

  • Sample throughput: High
  • Targets per sample: 10 to 130
  • NGS compatibility: Compatible with all Illumina Sequencers
  • Equipment required: Standard 96- or 384-well thermal cycler, no other equipment required; Pixel system is optional (Absolute Efficiency Standards)

mRNA

  • Sample throughput: High
  • Targets per sample: 10 to 130
  • NGS compatibility: Compatible with all Illumina Sequencers
  • Equipment required: Standard 96- or 384-well thermal cycler, no other equipment required; Pixel system is optional (Absolute Efficiency Standards)

Absolute Efficiency Standards -
Pixel RNA Spike-In

The Pixel™ system combined with Molecularly Indexed set of synthetic RNA standards enables users to determine the exact efficiency of a process such as standard RNA-seq or Precise assays. By simply counting the number of uniquely labeled molecules in the final library, the user can easily calculate the efficiency of the entire process. The method is ideally suited for sample quality control and process quality control application where it’s important to validate library quality before sequencing.

  • Ideal applications: RNA-seq, Precise assay quality control and determining the exact efficiency of a process
  • Sample throughput: 16 to 48
  • NGS compatibility: Compatible with all Illumina sequencers
  • Equipment required: Standard 96- or 384- well thermal cycler, no other equipment required

mRNA Absolute Quantification

The Pixel system is designed for quantifying gene expression in applications requiring precise and accurate quantitation of mRNA transcripts. Users can quantify expression of several genes in dozens of samples. The system is particularly well suited for applications requiring monitoring of very small changes in gene expression (< 10 fold change), and involving limited amounts of sample material when qPCR measurements become unreliable (< 100pg). The system is also an ideal alternative for any PCR-based quantification method exhibiting PCR bias as a result of differences in PCR assay efficiency.

  • Ideal applications: RNA-seq, Precise assay quality control and determining the exact efficiency of a process
  • Sample throughput: 16 to 48
  • NGS compatibility: Compatible with all Illumina sequencers
  • Equipment required: Standard 96- or 384- well thermal cycler, no other equipment required

Summary

Product # of Samples # of Targets Hardware Required
Pixel 10­ - 100 1 - 16 Pixel System, Thermal Cycler and Hybridization Oven
Precise 96­ - 10,000 10­ - 130 Thermal Cycler

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